JENNERIAN PROPHYLAXIS BY MEANS OF IN: TRADERMAL INJECTIONS OF CULTURE VACCINE VIRUS Bx
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چکیده
The successful cultivation of vaccine virus in a medium consisting of minced chick embryo tissue suspended in Tyrode's solution and the use of this virus by means of dermal inoculation for Jennerian prophylaxis in man have been described in previous communications (1, 2). A continuation of investigations of culture vaccine virus has rendered possible at this time a statement regarding the desirability of its intradermal use in man and a presentation of facts pertaining to its preservation and application. In an earlier report (2) the fact that the titer of our vaccine gradually diminished during the course of successive passages in the medium used was recorded. At that time there was also described a strain of culture virus, designated as the second revived strain, which maintained a constant titer and appeared to have become adapted to in vitro cultivation. This second revived strain has now been carried through 130 successive passages in chick embryo tissue and Tyrode's solution during a period of 3 years and still regularly yields when injected intradermally into rabbits typical mild vaccinai lesions. I t is assumed, therefore, that serial passage in cultures may be continued much longer, or that by reversion to earlier cultures preserved at low temperatures the necessity of further rejuvenation by passage through animals will seldom arise.
منابع مشابه
Jennerian Prophylaxis by Means of Intradermal Injections of Culture Vaccine Virus
The second revived strain of culture vaccine virus has been propagated through 130 culture passages during a period of 3 years. It seems to be adapted to in vitro cultivation and still has an intradermal titer (rabbits) of 1 to 100,000 or 1 to 1,000,000. Intradermal inoculations in human beings of 0.1 cc. amounts of culture virus diluted from 2.5 to 10 times result in primary takes in unvaccina...
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تاریخ انتشار 2003